Application of PSD-MALDI-TOF-MS to protein identification / 药学学报

ABSTRACT

<p><b>AIM</b>To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.</p><p><b>METHODS</b>The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.</p><p><b>RESULTS</b>Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.</p><p><b>CONCLUSION</b>PSD technique has greater application prospects in peoteomics.</p>