Study on methods for quality control of recombinant human neu epitope peptide 12 / 药学学报
Acta Pharmaceutica Sinica
;
(12): 359-362, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-302809
ABSTRACT
<p><b>AIM</b>To establish methods and requirements for the quality control of recombinant human neu epitope peptide 12.</p><p><b>METHODS</b>Biological activity of recombinant human neu epitope peptide 12 was evaluated in FVB/N transgenic mice (TgN MMTV neu 202 Mul, Jackson Lab., USA) administered with samples. The percentage of antibody-positive mice detected by ELISA was used in the biological activity evaluation. The peptide map was performed by peptic digestion. The antigen content was determined by SEC-HPLC.</p><p><b>RESULTS AND CONCLUSION</b>The quality control methods, such as biological activity, peptide map, antigen content, and the requirements for the quality control of recombinant human neu epitope peptide 12 were established. The established methods and requirements were already used for the quality control of recombinant human neu epitope peptide 12 products.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Fragments peptidiques
/
Contrôle de qualité
/
Normes de référence
/
Biotechnologie
/
Cartographie peptidique
/
Protéines de fusion recombinantes
/
Souris transgéniques
/
Chimie
/
Technologie pharmaceutique
/
Récepteur ErbB-2
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2004
Type:
Article
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