Development of a novel in-vitro culture device simulating distraction osteogenesis and expression of osteoblast cytoskeleton / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 111-113, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-303418
ABSTRACT
<p><b>OBJECTIVE</b>To establish a novel in-vitro culture device simulating distraction osteogenesis, and to understand the the changes of cytoskeleton of osteoblasts under different magnitudes of mechanical distraction.</p><p><b>METHODS</b>A novel in-vitro culture device simulating distraction osteogenesis was developed with titanium and silicon membrane. The expressions of F-actin cytoskeleton subjected to different magnitudes of distraction were observed in situ under a confocal laser scanning microscope, and protein levels of actin and tubulin alpha were analyzed by electrophoresis of Western blot.</p><p><b>RESULTS</b>The mode of culture device simulating distraction osteogenesis was similar to that of distraction osteogenesis used in clinic. After 24 h mechanical distraction with physiological magnitude, F-actin of osteoblasts was organized and paralleled to the direction of distraction forces. After 24 h mechanical distraction with hyperphysiological magnitude, however, F-actin of osteoblasts became reorganized and disrupted, and protein levels of actin and tubulin alpha decreased.</p><p><b>CONCLUSIONS</b>The novel in vitro culture device simulating distraction osteogenesis is suitable for the study of distraction osteogenesis in vitro. Physiological mechanical distraction causes organization and rearrangement of actin cytoskeleton. However, hyperphysiological mechanical distraction results in reorganization and disruption of actin cytoskeleton.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Physiologie
/
Tubuline
/
Cytosquelette
/
Cellules cultivées
/
Actines
/
Ostéogenèse par distraction
/
Biologie cellulaire
/
Modèles biologiques
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Stomatology
Année:
2006
Type:
Article
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