Effect of sensory neuropeptide substance P on the differentiation of cultured epidermal stem cells in vitro / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 295-298, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-303727
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of sensory neuropeptide substance P (SP) on the differentiation of cultured epidermal stem cells (ESC) in vitro,with in vitro cultured ESC as the platform.</p><p><b>METHODS</b>ESC from newborn Wistar rats were isolated, purified by repeated passages in culture. SP was added for stimulation when ESC clone grew. Immunohistochemistry staining with K14 antibody, and flow cytometry (FCM) was performed at 0, 24th, 48th, 72nd, 96th, 144th, 192nd, 240th, 288th, 336th, 384th, 432nd post differentiation hours (PDH) to identify the cell groups and to detect if there were transient amplifying cells (TAC) among the cells.</p><p><b>RESULTS</b>ESC in culture formed large colonies after SP treatment with positive staining for K14, indicating that they were TACs. The results of FCM indicated that when ESC were stimulated by SP, TAC colony formation occurred and the cell number increased in a constant speed.</p><p><b>CONCLUSION</b>ESC could differentiate into TAC by neuropeptide SP induction, and the number of ESC kept on a certain level during the process.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Cellules réceptrices sensorielles
/
Cellules souches
/
Substance P
/
Différenciation cellulaire
/
Cellules cultivées
/
Chimie
/
Rat Wistar
/
Biologie cellulaire
/
Cellules endothéliales
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Burns
Année:
2004
Type:
Article
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