Effect of topotecan on MAGE gene expression of HPB-AM cells / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 679-682, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-304617
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether topotecan, a novel anti-tumor agent, down-regulates gene expression of melanoma antigen-encoding (MAGE) in HPB-AM cells.</p><p><b>METHODS</b>MAGE mRNA expression of HPB-AM cells was detected by RT-PCR 4, 8, 12 and 16 hrs after different concentrations (0.05, 0.10, 0.15 and 0.20 micromol/L) of topotecan treatment.</p><p><b>RESULTS</b>MAGE mRNA expression of HPB-AM cells decreased with increasing concentrations of topotecan 12 hrs after treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10, 0.15 and 0.20 micromol/L of topotecan was significantly lower than that in the blank control group (P<0.05). MAGE mRNA expression of HPB-AM cells was significantly reduced in a time-dependent manner after 0.10 micromol/L of topotecan treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10 micromol/L of topotecan was significantly lower than that in the blank control group 12 and 16 hrs after treatment (P<0.05).</p><p><b>CONCLUSIONS</b>Topotecan is capable of inhibiting the expression of MAGE mRNA of HPB-AM cells in a time- and dose-dependent manner.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
ARN messager
/
Expression des gènes
/
Topotécane
/
Lignée cellulaire tumorale
/
Relation dose-effet des médicaments
/
Traitement médicamenteux
/
Génétique
/
Lymphomes
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Contemporary Pediatrics
Année:
2009
Type:
Article
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