Your browser doesn't support javascript.
loading
Hepatitis A virus mimotope mapping by phage display peptide library / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 456-459, 2012.
Article Dans Chinois | WPRIM | ID: wpr-305010
ABSTRACT
<p><b>OBJECTIVE</b>A 12 mer phage display peptide library was used to identify hepatitis A virus mimotopes of antigenic determinants, to provide the feasibility of virus epitope mapping by using this approach.</p><p><b>METHODS</b>Using purified anti-hepatitis A virus monoclonal antibody as affinity selective molecule, phage display peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.</p><p><b>RESULTS</b>10 ELISA positive clones were chosen for DNA sequencing, and the displayed peptide sequences were deduced. 9 of them showed identical nucleotide sequence, and similarity in their amino acid sequence with VP1 of HAV HM175 was found, but no sequence homology was found between the other phage clone and the capsid proteins of HAV. Those peptides may behave as mimotopes of HAV.</p><p><b>CONCLUSION</b>The mimotope of HAV was selected by using phage display peptide library screening. The results provide the potential of this method to search for the mimotopes of the virus.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Virologie / Données de séquences moléculaires / Chimie / Séquence d&apos;acides aminés / Cartographie épitopique / Banque de peptides / Virus de l&apos;hépatite A / Allergie et immunologie / Génétique / Hépatite A Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Experimental and Clinical Virology Année: 2012 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Virologie / Données de séquences moléculaires / Chimie / Séquence d&apos;acides aminés / Cartographie épitopique / Banque de peptides / Virus de l&apos;hépatite A / Allergie et immunologie / Génétique / Hépatite A Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Experimental and Clinical Virology Année: 2012 Type: Article