Establishment of confirmatory test for suspicious hepatitis B surface antigen positive samples / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 310-312, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-305049
ABSTRACT
<p><b>OBJECTIVE</b>Establish a confirmatory test based on ELISA, and use to verify the authenticity of HBsAg weak positive samples, pick and get rid of the false result, and avoid the mistake diagnosis.</p><p><b>METHOD</b>The particles (reagent A) coated by streptavidin and biotinylated HBsAb (reagent B) were mixed in different proportions, then neutralized with serum whose the COI of HBsAg > 20 by ELISA in order to identify the activity of HBsAb in confirmatory reagent. 30 pieces of HBsAg weak positive serum neutralized with the confirmatory reagent, the serum were considered to be positive if rate of decline of HBsAg COI > 50%. The results were compared to Roche confirmatory Kit.</p><p><b>RESULT</b>Confirmatory reagent was able to neutralized with HBsAg. 24 of 30 pieces of HBsAg weak positive samples were judged to be positive, while 6 poeces were negative. The ELISA comfirm method is fully consistent with Roche confirmatory Kit.</p><p><b>CONCLUSION</b>The ELISA confirmatory test for suspicious HBsAg positive samples is a simple, accurate and low cost initial validation method, After further clinical trials, should be widely applied.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Sang
/
Test ELISA
/
Diagnostic
/
Hépatite B
/
Anticorps de l'hépatite B
/
Antigènes de surface du virus de l'hépatite B
/
Méthodes
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2012
Type:
Article
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