Co-expression of gpd1 and hor2 from Saccharomyces cerevisiae in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 385-389, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-305264
ABSTRACT
Based on the principle of the pathway engineering, a novel pathway of producing glycerol was built in E. coli. The gpd1 gene encoding glycerol 3-phosphate dehydrogenase and the hor2 gene encoding glycerol 3-phosphatase were cloned from Saccharomyces cerevisiae, respectively. The two genes were inserted into expression vector pSE380 together. A recombinant plasmid pSE-gpd1-hor2 containing polycistron was constructed under the control of the strong trc promoter. Then it was transformed into E. coli BL21. The result showed the recombinant microorganism GxB-gh could convert glucose to glycerol directly. And the recombinant microorganism GxB-gh was incubated to produce glycerol from D-glucose in the fermentor. The maximal concentration of glycerol was 46.67g/L at 26h. Conversion rate of glucose was 42.87%. The study is about "green" producing glycerol by recombinant microorganism and is also useful for further working in recombining microorganism of producing 1,3-propanediol.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Saccharomyces cerevisiae
/
Protéines fongiques
/
Génie génétique
/
Clonage moléculaire
/
Phosphoric monoester hydrolases
/
Escherichia coli
/
Fermentation
/
Génétique
/
Glycérol
/
Glycerolphosphate dehydrogenase
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2005
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS