Detection of pathogens causing genital ulcer disease by multiplex polymerase chain reaction / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 273-275, 2005.
Article
Dans Anglais
| WPRIM
| ID: wpr-305402
ABSTRACT
<p><b>OBJECTIVE</b>To establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD).</p><p><b>METHODS</b>Based on the gene-specific region of the following pathogens Chlamydia trachomatis omp1/ompb, herpes simplex virus (HSV) DNA polymerase, Treponema pallidum tpp47, Haemophilus ducreyi 16s rRNA, four sets of primers were designed and an M-PCR assay was developed to detect four pathogens in one test. The assay was evaluated with diagnostic result of golden standard for each pathogen.</p><p><b>RESULTS</b>Of the 51 clinical samples, M-PCR showed slightly higher positive rate (47.1%) of HSV than cell culture (23.6%). Meanwhile, the positive rate of T. pallidum detected by M-PCR and dark-field microscopy was 19.6% (10/51) and 15.7% (8/51), respectively. Only one sample was positive for H. ducreyi and no sample was positive for C. trachomatis detected by both M-PCR assay and culture.</p><p><b>CONCLUSION</b>This primary study indicated that M-PCR assay can simultaneously and rapidly detect the four etiologic pathogens causing GUD.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Treponema pallidum
/
Ulcère
/
Virologie
/
Maladies sexuellement transmissibles
/
Chlamydia trachomatis
/
Haemophilus ducreyi
/
Réaction de polymérisation en chaîne
/
Herpèsvirus humain de type 2
/
Herpèsvirus humain de type 1
/
Amorces ADN
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Chinese Medical Sciences Journal
Année:
2005
Type:
Article
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