Development of methods for detection of H5N1 from human clinical specimens / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 24-26, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-305534
ABSTRACT
<p><b>BACKGROUND</b>To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.</p><p><b>METHODS</b>The typing and sub-typing primers were designed according to M and H5 and N1 gene respectively, and the RT-PCR and real-time PCR were developed using these primers.</p><p><b>RESULTS</b>The RT-PCR and real-time PCR could be used for H5N1 detection specifically, and there was no cross reaction with other influenza subtypes such as H1 and H3. The sensitivity for RT-PCR and real-time PCR was 1 TCID50 and 0.01 TCID50 respectively. Thirteen laboratory confirmed human H5N1 cases were detected from 42 suspected cases by using these methods.</p><p><b>CONCLUSION</b>The established RT-PCR and real-time PCR methods can be used for laboratory detection of suspected human H5N1 cases.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines virales
/
Virologie
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
Amorces ADN
/
RT-PCR
/
Diagnostic
/
Grippe humaine
/
Sous-type H5N1 du virus de la grippe A
/
Génétique
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2006
Type:
Article
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