Your browser doesn't support javascript.
loading
Development of methods for detection of H5N1 from human clinical specimens / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 24-26, 2006.
Article Dans Chinois | WPRIM | ID: wpr-305534
ABSTRACT
<p><b>BACKGROUND</b>To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.</p><p><b>METHODS</b>The typing and sub-typing primers were designed according to M and H5 and N1 gene respectively, and the RT-PCR and real-time PCR were developed using these primers.</p><p><b>RESULTS</b>The RT-PCR and real-time PCR could be used for H5N1 detection specifically, and there was no cross reaction with other influenza subtypes such as H1 and H3. The sensitivity for RT-PCR and real-time PCR was 1 TCID50 and 0.01 TCID50 respectively. Thirteen laboratory confirmed human H5N1 cases were detected from 42 suspected cases by using these methods.</p><p><b>CONCLUSION</b>The established RT-PCR and real-time PCR methods can be used for laboratory detection of suspected human H5N1 cases.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Protéines virales / Virologie / Reproductibilité des résultats / Sensibilité et spécificité / Amorces ADN / RT-PCR / Diagnostic / Grippe humaine / Sous-type H5N1 du virus de la grippe A / Génétique Type d'étude: Etude diagnostique Limites du sujet: Animaux / Humains langue: Chinois Texte intégral: Chinese Journal of Experimental and Clinical Virology Année: 2006 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Protéines virales / Virologie / Reproductibilité des résultats / Sensibilité et spécificité / Amorces ADN / RT-PCR / Diagnostic / Grippe humaine / Sous-type H5N1 du virus de la grippe A / Génétique Type d'étude: Etude diagnostique Limites du sujet: Animaux / Humains langue: Chinois Texte intégral: Chinese Journal of Experimental and Clinical Virology Année: 2006 Type: Article