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Isolation and identification of arboviruses in Hebei Province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 52-55, 2006.
Article Dans Chinois | WPRIM | ID: wpr-305546
ABSTRACT
<p><b>BACKGROUND</b>To study the arboviruses carried by mosquitoes collected in Hebei Province.</p><p><b>METHODS</b>Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR.</p><p><b>RESULTS</b>Totally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus.</p><p><b>CONCLUSION</b>Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phylogenèse / Arbovirus / Virologie / Analyse de regroupements / Lignée cellulaire / Classification / Analyse de séquence d&apos;ADN / RT-PCR / Génétique / Culicidae Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Experimental and Clinical Virology Année: 2006 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phylogenèse / Arbovirus / Virologie / Analyse de regroupements / Lignée cellulaire / Classification / Analyse de séquence d&apos;ADN / RT-PCR / Génétique / Culicidae Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Experimental and Clinical Virology Année: 2006 Type: Article