The role of tissue factor in the invasion and metastasis of colorectal carcinoma cells / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of tissue factor (TF) in the invasion and hematogenous metastasis of human colorectal carcinoma cells.</p><p><b>METHODS</b>The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 and LoVo cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western Blot. Then the transfected and un-transfected tumor cells were implanted into nude mice (Balb/c Nu/Nu) to produce primary tumor, lung metastasis and liver metastasis respectively.</p><p><b>RESULTS</b>HT-29 and LoVo cells with sense-TFcDNA transfection showed increased TF expression compared with the cells without transfection, but the cells with antisense-TFcDNA transfection got the contrary change. The primary tumor growth and invasive range, lung metastasis and live metastasis all increased in sense transfectants but reduced in antisense transfectants.</p><p><b>CONCLUSIONS</b>TF can increase the invasion and hematogenous metastatic ability of human colorectal carcinoma cells.</p>