Radiosensitivity on colorectal neoplasms by recombinant adenoviral-mediated wild-type p53 gene / 中华外科杂志
Chinese Journal of Surgery
; (12): 1002-1005, 2005.
Article
de Zh
| WPRIM
| ID: wpr-306148
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the radiosensitization on the cells of colorectal cancer transfected with recombinant adenovirus vector-mediated wild-type p53.</p><p><b>METHODS</b>SW480 cells transfected by wild-type p53 were treated with 4 Gy and 6 Gy radiation. The expression of recombinant adenovirus vector-mediated wild-type p53 gene was detected by Western blotting. The inhibition rate of SW480 cells was examined by MTT, apoptotic rate by TdT-mediated dUTP nick end labeling (TUNEL) and proliferating cell nuclear antigen (PCNA) by immunohistochemical method.</p><p><b>RESULTS</b>SW480 cells transfected by wild-type p53 were inhibited significantly by 4 Gy and 6 Gy radiation. The level of apoptosis increased and the expression of PCNA decreased.</p><p><b>CONCLUSION</b>Cells of colorectal carcinoma transfected with wild-type p53 increases their radiation sensitivity.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Effets des rayonnements
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Radiotolérance
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Radiothérapie
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Cellules cancéreuses en culture
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Transfection
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Tumeurs colorectales
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Adenoviridae
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Gènes p53
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Vecteurs génétiques
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Surgery
Année:
2005
Type:
Article