The Characterization of T Cell - Derived Neutrophil Survival Factor % / 대한면역학회지
Korean Journal of Immunology
;
: 363-374, 1997.
Article
Dans Coréen
| WPRIM
| ID: wpr-30626
ABSTRACT
It has been reported that the stimulated T lymphocytes might secret a neutrophil survival factor. Thus the goal of study was to determine which molecules are the neutrophil survival factors secreted from the phytohaemagglutinin (PHA)-stimulated T lymphocytes. Human peripheral blood T lymphocytes and neutrophils were isolated by Ficoll-paque density sedimentation from heparinized blood of healthy adult donors. The purity of T lymphocytes and neutrophils were more than 90% and 95%, respectively. The maximal effective condition for the neutrophil viability-sustaining activity was 1 ug/ml af PHA in 12 hours incubation with T lymphocytes. The effect of PHA-stimulated T lymphocyte conditioned medium (TCM) on the neutrophils were used for the comparison with PHA-nonstimulated TCM or enriched medium alone. Neutrophil viability-sustaining activity with PHA-stimulated TCM for 24 hours incubation was significantly higher than other groups (85+/-11 vs 43+/-5 vs 916%; p<0.01). In the analysis of the primary data, the good candidates for the neutrophil viability-sustaining factor were granulocyte/monocyte colony stimulating factor (GM-CSF) and interleukin-8 (IL-8). They were used in the bioassay and antibody neutralization of cytokine activity. ...continue...
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Donneurs de tissus
/
Dosage biologique
/
Héparine
/
Lymphocytes
/
Lymphocytes T
/
Facteur de stimulation des colonies de granulocytes et de macrophages
/
Interleukine-8
/
Facteurs de stimulation des colonies
/
Milieux de culture conditionnés
/
Granulocytes neutrophiles
Limites du sujet:
Adulte
/
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Immunology
Année:
1997
Type:
Article
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