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Identification of Up-Regulated Genes in Malignant Glioma with Subtraction Hybridization: Preliminary Screening Studies
Yonsei Medical Journal ; : 301-310, 2008.
Article Dans Anglais | WPRIM | ID: wpr-30670
ABSTRACT

PURPOSE:

This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. MATERIALS AND

METHODS:

Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank(TM) database.

RESULTS:

Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line.

CONCLUSION:

Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs du cerveau / ARN messager / Régulation de l'expression des gènes tumoraux / Régulation positive / RT-PCR / Lignée cellulaire tumorale / Gliome Type d'étude: Etude diagnostique / Étude de dépistage Limites du sujet: Adulte très âgé / Femelle / Humains / Mâle langue: Anglais Texte intégral: Yonsei Medical Journal Année: 2008 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs du cerveau / ARN messager / Régulation de l'expression des gènes tumoraux / Régulation positive / RT-PCR / Lignée cellulaire tumorale / Gliome Type d'étude: Etude diagnostique / Étude de dépistage Limites du sujet: Adulte très âgé / Femelle / Humains / Mâle langue: Anglais Texte intégral: Yonsei Medical Journal Année: 2008 Type: Article