Identification of Up-Regulated Genes in Malignant Glioma with Subtraction Hybridization: Preliminary Screening Studies
Yonsei Medical Journal
;
: 301-310, 2008.
Article
Dans Anglais
| WPRIM
| ID: wpr-30670
ABSTRACT
PURPOSE:
This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. MATERIALS ANDMETHODS:
Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank(TM) database.RESULTS:
Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line.CONCLUSION:
Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Tumeurs du cerveau
/
ARN messager
/
Régulation de l'expression des gènes tumoraux
/
Régulation positive
/
RT-PCR
/
Lignée cellulaire tumorale
/
Gliome
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Yonsei Medical Journal
Année:
2008
Type:
Article
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