Effect of trichostatin A and paclitaxel on the growth and apoptosis of lung adenocarcinoma cell lines / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 492-496, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-307355
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of trichostatin A (TSA)/paclitaxel on the growth and apoptosis in human lung adenocarcinoma cell line A549 cells.</p><p><b>METHODS</b>Human lung adenocarcinoma A549 cells were cultured in DMEM in the presence of paclitaxel and the histone deacetylase inhibitor trichostatin A, and the growth curve was obtained by trypan-blue exclusion assay and cell count. Apoptosis was assessed using Hoechst 33258 staining and flow cytometry, and cell cycle was detected by flow cytometry analysis. The proteins of PARP, caspase-3, survivin and tubulin acetylation were detected by Western blotting.</p><p><b>RESULTS</b>Significant growth reduction was observed in the A549 cells following treatment with paclitaxel or the histone deacetylase inhibitor TSA. The combined treatment with TSA/paclitaxel caused the highest inhibition of cell growth. The apoptosis rate of A549 cells treated with TSA or paclitaxel for 24 hours was (17.6 ± 1.8)% and (39.2 ± 3.7)%, respectively, but a significantly higher apoptosis rate was (64.2 ± 4.2)% was induced by combined treatment with TSA and paclitaxel. In contrast with the control group, the cell cycle was markedly arrested at G2/M phase in the TSA and paclitaxel group (P < 0.05). The Western blot analysis demonstrated that treatment with TSA/paclitaxel led to a synergistic increase of acetylated tubulin, PARP and caspase-3, and reduced the expression of survivin.</p><p><b>CONCLUSION</b>TSA or paclitaxel alone can inhibit the cell growth and induce apoptosis, and the combination of TSA and paclitaxel exerts a synergistic effect on the growth and apoptosis in lung adenocarcinoma cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Acétylation
/
Tubuline
/
Adénocarcinome
/
Cycle cellulaire
/
Poly(ADP-ribose) polymerases
/
Paclitaxel
/
Apoptose
/
Lignée cellulaire tumorale
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2012
Type:
Article
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