Simultaneous determination of 5 active components in Fructus Cnidii by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1883-1885, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-307570
ABSTRACT
<p><b>OBJECTIVE</b>To develop an RP-HPLC method for simultaneous determination of 5 constituents in Fructus Cnidii.</p><p><b>METHOD</b>Analysis was performed on an Alltech C18 (4.6 mm x 250 mm, 5 microm) column. The mobile phases were acetonitrile water and acetic acid with gradient elution. The flow rate was 1 mL x min(-1). The monitoring wavelength was 325 nm and 245 nm. The column temperature was 40 degrees C.</p><p><b>RESULT</b>The linear response ranges were 1-20 microg x mL(-1) (r = 0.999 9) for xanthotoxin, 1-20 microg x mL(-1) (r = 0.999 9) for isopimpinellin, 11-20 microg x mL(-1) (r = 0.999 8) for bergapten, 100-1 200 microg x mL(-1) (r = 0.999 7) for imperatorin, 100-2 000 microg x mL(-1) (r = 0.999 9) for osthole. The average recoveries were all above 95%.</p><p><b>CONCLUSION</b>The method is simple, sensitive and accurate with good reproducibility.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plantes médicinales
/
Furocoumarines
/
Chimie
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Coumarines
/
Cnidium
/
Fruit
/
Méthodes
/
Méthoxsalène
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2007
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS