Comparative analysis of the complete mitochondrial genome between Tibetan and Han population / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 382-386, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-308058
ABSTRACT
<p><b>OBJECTIVE</b>To construct the haplogroup and perform an analysis of mitochondrial whole-genome sequence in Tibetan and Han Chinese. Variations of nucleotide of mitochondrial DNA (mtDNA) were identified and compared between the Tibetan and Han population.</p><p><b>METHODS</b>The mtDNA whole sequences of 40 Tibetan and 50 Han individuals were sequenced by an Applied Biosystems 3730 DNA automatic sequencer. The sequences were assembled using software phredPhrap16.0, and all assembled sequences were manually verified according to the criterion of rCRS (revised Cambridge Reference Sequence). The haplogroups of mtDNA were constructed using phylogenetic analysis according to the criteria of MITOMAP by Network method. The data were elucidated by integrated methods.</p><p><b>RESULTS</b>Authors' results showed that all the pooled 90 subjects belonged to the Macrohaplogroup M and N, and were classified into 13 haplogroups. No differences were observed among the haplogroups of the two populations except for M9 haplogroup. A total of 21 variants were detected by comparing the mtDNA whole sequences between Tibetan and Han population; of those, 5 variants have not been reported before. In addition, we constructed the haplotypes of 5 variants harboring the D-loop region, and founded prominent difference in both supertype 1 and supertype 2 between Tibetan and Han population.</p><p><b>CONCLUSION</b>The phylogenetic analysis indicates that the Tibetan and Han ethnic groups shared close maternal relationship in origin. The biological implication of the significant variants is worth elucidating; whether they are the results of adaptive selection or neutral selection or pathological variations need to be further studied.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Haplotypes
/
ADN mitochondrial
/
Ethnies
/
Chine
/
Tibet
/
Évolution moléculaire
/
Asiatiques
/
Ethnologie
/
Génome mitochondrial
/
Génétique
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2008
Type:
Article
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