Assessment of polymerase chain reaction and serology for detection of chlamydia pneumoniae in patients with acute respiratory tract infection / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 184-187, 2002.
Article
de En
| WPRIM
| ID: wpr-308123
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study Chlamydia pneumoniae (C. pneumoniae) infection in 110 patients with respiratory tract infection admitted to our hospital from January to December 1995 in Nanjing.</p><p><b>METHODS</b>Sputum and throat swab specimens were taken and C. pneumoniae DNA was detected by using polymerase chain reaction (PCR) with the HM-1-HR-1 primer pair. At the same time, serum samples were taken and immunoglobulin G and M (IgG and IgM) fractions of antibodies to C. pneumoniae were studied by microimmunofluorescence test.</p><p><b>RESULTS</b>Prevalence of specific IgG was 70% in patients with respiratory tract infection. Seventeen patients (15.5%) were serologically diagnosed as having recent C. pneumoniae infections and 12 patients (10.9%) had positive PCR in sputum and/or swab specimens. The total positive rate was 22.7% (25/110) detected by PCR combined with serological tests. Acute infection of C. pneumoniae was common in patients with asthma (57.1%), pneumonia (35.0%), COPD (25.9%) and bronchitis (25.0%). Clinical features between C. pneumoniae infection and non-C. pneumonia infection showed no significant differences.</p><p><b>CONCLUSIONS</b>Chlamydia pneumoniae is an important pathogen that causes infection of the human respiratory tract and attention should be drawn to this special illness.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sang
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ADN bactérien
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Immunoglobuline G
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Réaction de polymérisation en chaîne
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Maladie aigüe
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Facteurs âges
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Chlamydophila pneumoniae
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Pneumopathie bactérienne
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Allergie et immunologie
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Génétique
Type d'étude:
Diagnostic_studies
Limites du sujet:
Adolescent
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Adult
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Aged
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Aged80
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Female
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Humans
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Male
langue:
En
Texte intégral:
Chinese Medical Journal
Année:
2002
Type:
Article