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Protective effect of qingxin kaiqiao fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35 / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 3271-3274, 2012.
Article Dans Chinois | WPRIM | ID: wpr-308602
ABSTRACT
<p><b>OBJECTIVE</b>To observe the protective effect of Qingxin Kaiqiao Fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35, in order to provide basis for clinical application of the formula.</p><p><b>METHOD</b>Sprague Dawley rats were orally administration with Qingxin Kaiqiao Fang (7.9 g x kg(-1)) twice a day for 3.5 days to prepare Qingxin Kaiqiao Fang containing cerebrospinal fluid. The nerve cell injury model was established by PC12 cells and Abeta25-35 with the concentration of 10 micromol x L(-1). The expressions of Bax, Bcl-2 and Caspase-3 mRNA were detected by immunohistochemical method in the PC12 cells.</p><p><b>RESULT</b>The Qingxin Kaiqiao Fang group showed a significant higher PC12 cell activity than the model group, with decrease in Bax mRNA and Caspase-3 mRNA expressions and increase in Bcl-2 mRNA expression. There was a significant difference from the model group (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>Qinxin Kaiqiao Fang shows a significant protective effect on Abeta25-35-induced nerve cell injury.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Fragments peptidiques / Pharmacologie / Survie cellulaire / Liquide cérébrospinal / Peptides bêta-amyloïdes / Cellules PC12 / Rat Sprague-Dawley / Apoptose / Neuroprotecteurs / Protéines proto-oncogènes c-bcl-2 Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2012 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Fragments peptidiques / Pharmacologie / Survie cellulaire / Liquide cérébrospinal / Peptides bêta-amyloïdes / Cellules PC12 / Rat Sprague-Dawley / Apoptose / Neuroprotecteurs / Protéines proto-oncogènes c-bcl-2 Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2012 Type: Article