Effect of ptxA and ptxB genes of phosphotransferase system on growth of Streptococcus mutans / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 302-306, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-309133
ABSTRACT
<p><b>OBJECTIVE</b>This study aims to evaluate the effect of ptxA and ptxB genes, which are important genes in the L-ascorbate phosphotransferase system (PTS) of Streptococcus mutans (S. mutans).</p><p><b>METHODS</b>The ptxA-, ptxB-, and ptxAB-double deficient mutant as well as ptxAB-complemented strain were constructed. Quantitative real-time polymerase chain reaction analysis was performed to evaluate the expression of the target genes of wild-type S. mutans when L-ascorbate was used as the sole carbohydrate source. The OD₆₀₀ values of the wild type, deficient, and complemented strains were continuously monitored, and their growth curves were constructed to compare growth capacity.</p><p><b>RESULTS</b>Polymerase chain reaction and sequencing analyses suggested that deficient and complemented strains were successfully constructed. The expression levelsof ptxA and ptxB significantly increased (P < 0.01) when L-ascorbate was used as the sole carbohydrate source. The growth capacity of the deficient mutants decreased compared with that of the wild-type strain. However, the wild-type phenotype could be restored in the complemented strain.</p><p><b>CONCLUSION</b>ptxA and ptxB genes are associated with L-ascorbate metabolism of S. mutans. The construction of deficient strains and complemented strain lay a foundation for further mechanism study on L-ascorbate metabolism in S. mutans.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphotransferases
/
Physiologie
/
Streptococcus mutans
/
Protéines bactériennes
/
Facteurs de transcription
/
Réaction de polymérisation en chaine en temps réel
/
Gènes bactériens
/
Génétique
/
Métabolisme
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2016
Type:
Article
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