Growth factors promote the survival and proliferation of human spermatogonium stem cells in vitro / 中华男科学杂志
National Journal of Andrology
; (12): 876-878, 2008.
Article
de Zh
| WPRIM
| ID: wpr-309758
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells (SSCs) in vitro.</p><p><b>METHODS</b>SSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concentrations of 0, 5, 10 and 20 microg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope.</p><p><b>RESULTS</b>The survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control (P < 0.05).</p><p><b>CONCLUSION</b>The growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Spermatogonies
/
Cellules souches
/
Survie cellulaire
/
Cellules cultivées
/
Facteur de croissance fibroblastique de type 2
/
Facteur de croissance des cellules souches
/
Biologie cellulaire
/
Protéines et peptides de signalisation intercellulaire
/
Prolifération cellulaire
Limites du sujet:
Humans
/
Male
langue:
Zh
Texte intégral:
National Journal of Andrology
Année:
2008
Type:
Article