The effect of hepatitis B virus X protein on the c-met promoter activity in HepG2 cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 292-296, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-310107
ABSTRACT
<p><b>OBJECTIVE</b>To confirm the effect of hepatitis B virus X (HBx) protein on the c-met promoter activity in HepG2 cells.</p><p><b>METHOD</b>The expression of c-met protein was detected by western blot in HBx-transfected HepG2 cells, the human c-met promote activity was checked by luciferase assay using five different constructs with deletion or point mutation.</p><p><b>RESULTS</b>HBx protein stimulated the expression of the c-met in HepG2 cells. The enhanced expression of c-met in HBx-transfected cells was mediated by the activation of AP-2 and SP-1 transcriptional activity at the c-met promoter region (-183bp--100bp), HBx increased the invasiveness of HepG2 cells as determined by Matrigel invasion assay.</p><p><b>CONCLUSION</b>These results suggests that HBx induces the expression of c-met through the activation of AP-2 and SP-1 activity at the promoter region; in addition, our data indicate that HBx stimulates the invasive potential of HepG2 cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Plasmides
/
Facteurs de transcription
/
Transfection
/
Transactivateurs
/
Régulation de l'expression des gènes
/
Virus de l'hépatite B
/
Technique de Western
/
Réaction de polymérisation en chaîne
/
Régions promotrices (génétique)
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hepatology
Année:
2009
Type:
Article
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