Peptide TAT modified polyethylenimine-beta-cyclodextrin for gene delivery / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 15-23, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-310396
ABSTRACT
<p><b>OBJECTIVE</b>To develop a novel gene delivery vector TAT-PEI-beta-CyD.</p><p><b>METHODS</b>beta-cyclodextrin (beta-CyD) was linked by low molecular weight (PEI 600) via 1, 1-carbonyldiimidazole (CDI), and TAT peptide (RRRQRRKKRC) was coupled to PEI 600 by [N-succinimidy-3-(2-pyridyldithio) propionate, SPDP]. The copolymer was characterized by (1)H-NMR and FT-IR. Physiochemical characteristics of TAT-PEI-beta-CyD/DNA complexes were tested by agarose gel electrophoresis and particle size measurements. Cell viability and transfection efficiency were evaluated in A293 and B16 cells using PEI 25 kDa as a control.</p><p><b>RESULT</b>TAT peptide was successfully coupled to PEI-beta-CyD. The result of gel electrophoresis showed that the TAT-PEI-beta-CyD was able to condense DNA efficiently at N/P ratio of 4. The particle size of TAT-PEI-beta-CyD/DNA complexes was around 100 nm. The cytotoxicity of TAT-PEI-beta-CyD was lower than that of PEI 25 kDa. The transfection efficiency of TAT-PEI-beta-CyD was higher than that of PEI 25 kDa in A293 and B16 cells at N/P ratio of 30.</p><p><b>CONCLUSION</b>The novel vector TAT-PEI-beta-CyD has been developed successfully with low cytotoxicity and high transfection efficiency.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Fragments peptidiques
/
Polyéthylèneimine
/
Thérapie génétique
/
Lignée cellulaire
/
Chimie
/
Techniques de transfert de gènes
/
Cyclodextrines bêta
/
Produits du gène tat du virus de l'immunodéficience humaine
/
Méthodes
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2009
Type:
Article
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