Establishment of animal model with B lineage acute leukemia in nude mice for evaluation of new therapeutic agents / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To establish an acute leukemia animal model for testing new therapeutic agents in vivo.</p><p><b>METHODS</b>Nude mice were intraperitoneally injected with 2 mg cyclophosphamide, 24 h later 5 x 10(6) acute B-cell leukemia Nalm-6 cells was inoculated via the tail vein, then monitored daily. When animals were paralyzed or dying, the organs including the liver, spleen, lung, heart, kidney, brain, bone marrow, pancreas, testes were removed and fixed with formalin, examined by routine histopathology.</p><p><b>RESULTS</b>After Nalm-6 cells were inoculated the mean survival of mice were( 19.4+/-0.55)d (n=6). The paralysis of mice was followed by weight loss, bent spines, hogback, cachexia and death. Histopathological examination showed that the tumor cells infiltrated liver, spleen, kidney, lung, meninges, interior cerebrum, the liver and kidney were the most affected organs.</p><p><b>CONCLUSION</b>B lineage acute leukemia animal model has been successfully established in the nude mice, which is suitable for testing new therapeutic agents.</p>