Cortical neuron apoptosis induced by beta-amyloid peptide and protective effect of panoxadiol in mice / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 451-455, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-310432
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis of cortical neurons induced by beta-amyloid peptide (Abeta(1-40)) and the protective effect of panoxadiol.</p><p><b>METHODS</b>The Abeta(1-40) induced damage of primarily cultured mouse cortical neurons was examined with morphological observation, MTT assay, DNA agarose gel electrophoresis and Western-blot.</p><p><b>RESULT</b>After 48 h treated with 12 mumol/L Abeta(1-40), the cortical neurons showed apoptotic characteristics including decreased OD570 value in MTT assay, DNA cleavage fragment in electrophoresis and increased apoptotic cells. Western-blot showed that the expression of bcl-2 reduced significantly (P<0.05). Cell apoptosis was significantly attenuated in 40 mg/L panoxadiol treated group.</p><p><b>CONCLUSION</b>Panoxadiol can protect cultured cortical neurons from apoptosis induced by Abeta(1-40) in mice.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Fragments peptidiques
/
Pharmacologie
/
Médicaments issus de plantes chinoises
/
Cellules cultivées
/
Cortex cérébral
/
Peptides bêta-amyloïdes
/
Apoptose
/
Neuroprotecteurs
/
Protéines proto-oncogènes c-bcl-2
/
Biologie cellulaire
Limites du sujet:
Animaux
/
Grossesse
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2008
Type:
Article
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