Effects of hypoxic preconditioning on hypoxia tolerance of astrocytes in vitro / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To explore the mechanisms of hypoxic preconditioning on protecting cultured astrocytes from hypoxia injury.</p><p><b>METHODS</b>Cultured astrocytes were divided randomly into several groups: control(C), hypoxia(H) and hypoxic preconditioning (HP). Cells MTT metabolic activity, qualitation of apoptosis and modality to explore the protection effects of hypoxic preconditioning. Immunocytochemistry of Bcl-2 and Bax to explore the mechanisms of hypoxic preconditioning on protecting astrocytes from hypoxia.</p><p><b>RESULTS</b>Compared with H group there was marked increase of MTT metabolic activity in HP48 and HP72 groups. Immunocytochemistry of Bcl-2 and Bax showed that compared with H group, expression of Bcl-2 was increased in HP group, while expression of Bax was decreased in HP group.</p><p><b>CONCLUSION</b>Hypoxic preconditioning can protect astrocytes from hypoxia. One possible mechanism maybe concerned with inhibition of Bax and maintain of Bcl-2 to depress apoptosis procedure.</p>