Effects of hypoxic preconditioning on hypoxia tolerance of astrocytes in vitro / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 30-34, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-310811
ABSTRACT
<p><b>AIM</b>To explore the mechanisms of hypoxic preconditioning on protecting cultured astrocytes from hypoxia injury.</p><p><b>METHODS</b>Cultured astrocytes were divided randomly into several groups control(C), hypoxia(H) and hypoxic preconditioning (HP). Cells MTT metabolic activity, qualitation of apoptosis and modality to explore the protection effects of hypoxic preconditioning. Immunocytochemistry of Bcl-2 and Bax to explore the mechanisms of hypoxic preconditioning on protecting astrocytes from hypoxia.</p><p><b>RESULTS</b>Compared with H group there was marked increase of MTT metabolic activity in HP48 and HP72 groups. Immunocytochemistry of Bcl-2 and Bax showed that compared with H group, expression of Bcl-2 was increased in HP group, while expression of Bax was decreased in HP group.</p><p><b>CONCLUSION</b>Hypoxic preconditioning can protect astrocytes from hypoxia. One possible mechanism maybe concerned with inhibition of Bax and maintain of Bcl-2 to depress apoptosis procedure.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Adaptation physiologique
/
Hypoxie cellulaire
/
Cellules cultivées
/
Astrocytes
/
Rat Sprague-Dawley
/
Apoptose
/
Protéines proto-oncogènes c-bcl-2
/
Préconditionnement ischémique
/
Biologie cellulaire
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2008
Type:
Article
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