Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 431-437, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-310867
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether emodin exerts protective effects on mouse with allergic asthma.</p><p><b>METHODS</b>A mouse model of allergic airway inflflammation was employed. The C57BL/6 mice sensitized and challenged with ovalbumin (OVA) were intraperitoneally administered 10 or 20 mg/kg emodin for 3 days during OVA challenge. Animals were sacrificed 48 h after the last challenge. Inflammatory cell count in the bronchoalveolar lavage fluid (BALF) was measured. The levels of interleukin (IL)-4, IL-5, IL-13 and eotaxin in BALF and level of immunoglobulin E (IgE) in serum were measured with enzyme-linked immuno sorbent assay kits. The mRNA expressions of IL-4, IL-5, heme oxygenase (HO)-1 and matrix metalloproteinase-9 (MMP-9) were determined by real-time quantitative polymerase chain reaction.</p><p><b>RESULTS</b>Emodin induced significant suppression of the number of OVA-induced total inflammatory cells in BALF. Treatment with emodin led to significant decreases in the levels of IL-4, IL-5, IL-13 and eotaxin in BALF and total IgE level in serum. Histological examination of lung tissue revealed marked attenuation of allergen-induced lung eosinophilic inflammation. Additionally, emodin suppressed IL-4, IL-5 and MMP-9 mRNA expressions and induced HO-1 mRNA expression.</p><p><b>CONCLUSION</b>Emodin exhibits anti-inflammatory activity in the airway inflammation mouse model, supporting its therapeutic potential for the treatment of allergic bronchial asthma.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Pneumopathie infectieuse
/
Sang
/
Immunoglobuline E
/
ARN messager
/
Liquide de lavage bronchoalvéolaire
/
Chimie
/
Ovalbumine
/
Régulation de l'expression des gènes
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese journal of integrative medicine
Année:
2015
Type:
Article
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