HBx gene facilitates the proliferation activity of hepatoma cells in vitro and in vivo / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of HBx gene on proliferation activity of hepatoma cells in vitro and in vivo.</p><p><b>METHODS</b>The plasmid pHA-HBx carrying HBx gene was transfected into HepG(2) cells, and the positive clones were screened and identified with G418 and RT-PCR, respectively. The growth curve and population doubling time were calculated, and the cell cycle was analyzed by flow cytometry (FCM). The proliferation activity of transformed cells was measured with (3)H-TdR incorporation rate and nude mice model in vitro and in vivo.</p><p><b>RESULTS</b>The result of RT-PCR indicated that HBx gene was integrated into the genome DNA of HepG(2) cells and transcripted. The growth curve and population doubling time showed a high proliferation activity of transformed cells. The amount of cells at stage S and G(2)/M were significantly higher, and cells at stage G(0)/G(1) were lower than those in control group. The tumors developed from transfected cells grew much quicker than those developed from HepG(2) cells in nude mice model.</p><p><b>CONCLUSION</b>HBx gene can facilitate the proliferation of hepatoma cells both in vitro and in vivo.</p>