Examine the Correlation between Heat Shock Protein IbpA and Heat Tolerance in Cronobacter sakazakii / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 606-610, 2017.
Article
Dans Anglais
| WPRIM
| ID: wpr-311372
ABSTRACT
We used a proteomic approach to identify IbpA in Cronobacter sakazakii (C. sakazaki), which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C. sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 ΔibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 ΔibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coli O157H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Stress physiologique
/
Protéines bactériennes
/
Régulation de l'expression des gènes bactériens
/
Cronobacter sakazakii
/
Génétique
/
Génotype
/
Température élevée
/
Protéines du choc thermique
/
Métabolisme
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2017
Type:
Article
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