Unique GGT --> GTT mutation at K-ras codon 12 in six human pancreatic cancer cell lines from Chinese patients / 中华医学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients.</p><p><b>METHODS</b>All six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing.</p><p><b>RESULTS</b>All 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13.</p><p><b>CONCLUSION</b>The unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.</p>