Effect of gastric bypass surgery on mRNA expression level of hepatic phosphoenolpyruvate carboxykinase in type 2 diabetic Goto-Kakizaki rats / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 1146-1149, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-312330
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of gastric bypass surgery(GBP) on hepatic phosphoenolpyruvate carboxykinase(PEPCK) mRNA expression in type 2 diabetic Goto-Kakizaki rats.</p><p><b>METHODS</b>Male GK rats were randomized into three groups gastric bypass surgery(n=10), sham operation with diet restriction(n=10), and sham operation alone(n=10). Liver specimens of GK rats were collected during the intraoperative period for self-control study and 8 weeks after surgery. Fasting blood glucose, food intake, and body weight were recorded before surgery and 1, 2, 4, 8 weeks after surgery. The expression of PEPCK mRNA was measured by real-time PCR.</p><p><b>RESULTS</b>The fasting plasma glucose level decreased from(17.6±2.1) mmol/L before surgery to(7.5±0.9) mmol/L 8 weeks after surgery in GBP group. The level of PEPCK mRNA decreased from 1.08±0.38 before surgery to 0.41±0.10 8 weeks after surgery, significantly lower than that in sham operation alone group(1.04±0.12)(P<0.01). The level of PEPCK mRNA in diet restriction group increased from 1.15±0.16 before surgery to 2.54±0.82 8 weeks after surgery(P<0.01). The expression of PEPCK mRNA in diet restriction was significantly higher than that in CBP group(P<0.01).</p><p><b>CONCLUSIONS</b>GBP can significantly improve hyperglycemia in type 2 diabetic GK rat models, which may be associated with the decrease of hepatic PEPCK mRNA level.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphoenolpyruvate carboxykinase (GTP)
/
Chirurgie générale
/
Glycémie
/
ARN messager
/
Dérivation gastrique
/
Protéines et peptides de signalisation intracellulaire
/
Diabète expérimental
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Diabète de type 2
/
Génétique
/
Foie
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Gastrointestinal Surgery
Année:
2012
Type:
Article
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