Effect of dureping injection on T-cells function and their function in killing FM1 infected Mphi in vitro / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 729-732, 2010.
Article
de Zh
| WPRIM
| ID: wpr-313210
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Dureping Injection (DRP) on the T-cells function of mice and the function of T-cells in killing MF infected by influenza virus subtype A mice-lung adaptive strain FM1 in vitro.</p><p><b>METHODS</b>Number of splenic normal and FM1 infected T-cells in mice were measured by MTT and double-antibody sandwich ELISA, after being treated with DRP at different concentrations (2.1, 8.5 and 17.0 microg/mL), and the effect of DRP on interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) production as well as on splenic T-cell killing FM1 infected Mphi/Ana-1 function were detected.</p><p><b>RESULTS</b>DRP inhibited the multiplication of normal spleen T cells induced by concanavalin A in vitro, suppressed Th2 cell factor IL-10 production, and maintained Th1 cell factor IFN-y at a definite level, moreover, it directly enhanced the power of T-cells in killing FM1 infected Mphi (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>DRP could act on mice T-cells to enhance the immune response for antiinfluenza viral FM1 in vitro.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Antiviraux
/
Virus de la grippe A
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Pharmacologie
/
Rate
/
Virologie
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Médicaments issus de plantes chinoises
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Lymphocytes T
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Cellules cultivées
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Adjuvants immunologiques
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Interféron gamma
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Integrated Traditional and Western Medicine
Année:
2010
Type:
Article