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Expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 805-808, 2011.
Article Dans Chinois | WPRIM | ID: wpr-313890
ABSTRACT
This study was aimed to investigate the expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells (MSC). Human bone marrow MSC were isolated and enriched by Fircoll density gradient centrifugation, then identified by flow cytometry. MSC were induced with dexamethasone, insulin, 3-isobutyl-1-methyl-xanthine (IBMX) and indomethacin to differentiate into adipocytes. Then the expression of survivin and HO-1 in MSC was detected by RT-PCR. The results indicated that the expressions of surface antigen CD34 and HLA-DR in MSC in vitro were negative while the expressions of CD44 and CD71 were positive. MSC could be differentiated into adipocytes by inductor. RT-PCR showed the expression of radioresistant genes survivin and HO-1 in MSC. It is concluded that MSC have lower sensitivity to radiation, which may associate with the expression of radioresistant genes survivin and HO-1 in MSC.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Effets des rayonnements / Cellules de la moelle osseuse / Expression des gènes / Différenciation cellulaire / Cellules cultivées / Heme oxygenase-1 / Protéines IAP / Cellules souches mésenchymateuses / Cytométrie en flux / Génétique Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Experimental Hematology Année: 2011 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Effets des rayonnements / Cellules de la moelle osseuse / Expression des gènes / Différenciation cellulaire / Cellules cultivées / Heme oxygenase-1 / Protéines IAP / Cellules souches mésenchymateuses / Cytométrie en flux / Génétique Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Experimental Hematology Année: 2011 Type: Article