Effects of hyperbaric oxygen combined with adriamycin in inducing apoptosis of K562/A02 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 621-625, 2011.
Article
de Zh
| WPRIM
| ID: wpr-313930
Bibliothèque responsable:
WPRO
ABSTRACT
This study was purposed to explore the effects of hyperbaric oxygen (HBO) combined with adriamycin (ADM) on inducing apoptosis of multidrug resistant cells line K562/A02. The cell apoptosis and expression of caspase-3 activity were analyzed by flow cytometry and transmission electron microscopy; the expression levels of HIF-1α, BCL-2 and BAX mRNA were detected by quantitative real time PCR; the caspase 8 activity was determined by using caspase 8 kit; the expression level of P-gp was detected by Western blot. The results showed that the apoptosis rate of K562/A02 cells in combination group (0.2 MPa HBO + ADM) was higher than that in ADM group [(47.36 ± 3.87) % vs (28.51 ± 1.09) %], the difference was statistical significant (p < 0.05); the expression levels of HIF-1α mRNA, P-gp and BCL-2 in combination group were lower than those in ADM group, there were significant differences (p < 0.05); the activities of BAX, caspase 3 and caspase 8 proteins in combination group were higher than those in ADM group, the difference was statistical significant (p < 0.05). It is concluded that 0.2 MPa HBO combined with ADM can reverse the drug-resistance of K562/A02 cells to ADM, enhance the apoptosis rate of cells. The molecular mechanism may be related with down-regulation of P-gp and BCL-2 expression, and up-regulation of caspase-3 and caspase-8 activities by HIF-1α.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Doxorubicine
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Régulation de l'expression des gènes dans la leucémie
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Apoptose
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Glycoprotéine P
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Multirésistance aux médicaments
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Résistance aux médicaments antinéoplasiques
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Protéines proto-oncogènes c-bcl-2
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Cellules K562
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Sous-unité alpha du facteur-1 induit par l'hypoxie
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2011
Type:
Article