Expression of Total Vascular Endothelial Growth Factor and the Anti-angiogenic VEGF 165 b Isoform in the Vitreous of Patients with Retinopathy of Prematurity / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 2505-2509, 2015.
Article
de En
| WPRIM
| ID: wpr-315306
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>This study was to examine the expression of total vascular endothelial growth factor (VEGF) and the anti-angiogenic VEGF 165 b isoform in the vitreous body of retinopathy of prematurity (ROP) patients, and to further study the role of the VEGF splicing in the development of ROP.</p><p><b>METHODS</b>This was a prospective clinical laboratory investigation study. All patients enrolled received standard ophthalmic examination with stage 4 ROP that required vitrectomy to collect the vitreous samples. The control samples were from congenital cataract patients. The expression of total VEGF and the anti-angiogenic VEGF 165 b were measured by enzyme-linked immunosorbent assay. Results were analyzed statistically using nonparametric tests.</p><p><b>RESULTS</b>The total VEGF level was markedly elevated in ROP samples while VEGF 165 b was markedly decreased compared to control group. The relative protein expression level of VEGF 165 b isoform was significantly decreased in ROP patients which were correlated with the ischemia-induced neovascularization.</p><p><b>CONCLUSIONS</b>There was a switch of VEGF splicing from anti-angiogenic to pro-angiogenic family in ROP patients. A specific inhibitor that more selectively targets VEGF 165 and controls the VEGF splicing between pro- and anti-angiogenic families might be a more effective therapy for ROP.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Corps vitré
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Rétinopathie du prématuré
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Prématuré
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Test ELISA
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Études prospectives
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Isoformes de protéines
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Facteur de croissance endothéliale vasculaire de type A
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Métabolisme
Type d'étude:
Observational_studies
Limites du sujet:
Female
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Humans
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Male
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Newborn
langue:
En
Texte intégral:
Chinese Medical Journal
Année:
2015
Type:
Article