Cloning and characterization of open reading frame encoding beta-amyrin synthase in Glycyrrhiza uralensis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To clone and sequence the open reading frame of beta-amyrin synthase (bAS) from Glycyrrhiza uralensis.</p><p><b>METHOD</b>The primers were designed according to the cDNA sequence of beta-amyrin synthase from G. glabra reported by Hiroaki HAYASHI, and the open reading frame of beta-amyrin synthase was cloned by RT-PCR strategy with the template of total RNA extracted from roots of G. uralensis.</p><p><b>RESULT</b>The GubAS (GenBank Accession number: FJ627179) was 2 289 bp in length encoding one pelypeptide of 762 amino acid. Deduced amino acid sequence had 99%, 92%, 90%, 90% and 89% homology to the amino acid sequence of G. glabra, Lotus japonicus, Pisum sativum, Medicago truncatula, Glycine max, respectively.</p><p><b>CONCLUSION</b>The open reading frame of bAS from G. uralensis is cloned and reported for the first time. The conclusion will provide a foundation for exploring the mechanism of triterpenes biosynthesis.</p>