Effect of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension culture / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1580-1583, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-316003
ABSTRACT
<p><b>OBJECTIVE</b>To illustrate the effects of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension cultures.</p><p><b>METHOD</b>High performance liquid chromatography and plant tissue culture were applied.</p><p><b>RESULT AND CONCLUSION</b>Cell growth and isoflavone accumulation were significantly stimulated in P. lobata cell suspension cultures by the increase of the sucrose concentration. Maintaining the pH value at the range over 5. 4 to 5. 8 was most suitable for isoflavone accumulation in P. lobata cell suspension cultures. Cell dried weight and isoflavone accumulation decreased sharply with the increase of the treated concentration of active carbon, while XAD-4 significantly stimulated cell growth and isoflavone accumulation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Plantes médicinales
/
Polystyrènes
/
Polyvinyles
/
Saccharose
/
Cellules cultivées
/
Charbon de bois
/
Milieux de culture
/
Pueraria
/
Biologie cellulaire
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2006
Type:
Article
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