Distribution and effects of 5-HT(1A) receptors in distal cerebral spinal fluid-contacting neurons in rat brain parenchyma in neuropathic pain / 生理学报
Acta Physiologica Sinica
;
(6): 243-248, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-316734
ABSTRACT
The present study aimed to explore the effects of 5-HT(1A) receptors in the distal cerebral spinal fluid-contacting neurons (CSF-CNs) in rat brain parenchyma in neuropathic pain. The model of neuropathic pain with chronic constriction injury (CCI) of the sciatic nerve was made in Sprague-Dawley rats. The behavioral studies of animal were scored and the paw withdrawal latency (PWL) and paw withdrawal threshold (PWT) were measured. The distribution and expression of 5-HT(1A) receptors were observed in the distal CSF-CNs in brain parenchyma with double labeling of cholera toxin subunit B with horseradish peroxidase (CB-HRP) and 5-HT(1A) receptors with immunhistochemistry. The relationship between 5-HT(1A) receptors in distal CSF-CNs and neuropathic pain was analyzed. The results were as follows. On days 1, 3, 7, 14 of neuropathic pain, the PWL was 19.37±2.74, 12.04±1.77, 8.74±1.15 and 12.31±1.94, respectively; the PWT was 18.58±3.62, 13.05±1.81, 6.66±1.43 and 11.55±2.01, respectively. CB-HRP-labeled neurons of two clusters were always found in definite region but not in other area in brain parenchyma. The number of neurons double-labeled with CB-HRP/5-HT(1A) receptors in each group was 276.14±36.00, 161.72±28.41, 108.64±6.81, and 139.76±44.64, which was about 95%, 60%, 40% and 55% of all CB-HRP-labeled neurons in the four courses of neuropathic pain, respectively. It is suggested that the distal CSF-CNs are always located in a special region in rat brain parenchyma and most of them have 5-HT(1A) receptors. A negative correlation is found between the expression of 5-HT(1A) receptors and neuropathic pain.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Encéphale
/
Liquide cérébrospinal
/
Rat Sprague-Dawley
/
Biologie cellulaire
/
Récepteur de la sérotonine de type 5-HT1A
/
Névralgie
/
Neurones
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Physiologica Sinica
Année:
2008
Type:
Article
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