Establishment of localization ultrathin section for cytopathic cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 376-378, 2013.
Article
de Zh
| WPRIM
| ID: wpr-318015
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a localization ultrathin section method through which target cytopathic cells could be sectioned in situ.</p><p><b>METHODS</b>Lab-Tek Chamber slide system (177402) was selected as resin embedding mould. Cells infected with Human adenovirus type 5 (Ad5) or A/HN/SWL3/ 2009 (H1N1) influenza virus were embedded in situ as models. Target cytopathic cells were exposed by trimming, sectioned and observed under transmission electron microscope (TEM).</p><p><b>RESULTS</b>Target cells could be sectioned in situ and virus particles could be found easily on sections.</p><p><b>CONCLUSION</b>A localization ultrathin sectioning method was established and this technique could be applied in virus detection in cytopathic cells to improve TEM detection efficiency.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Physiologie
/
Virologie
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Lignée cellulaire
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Infections humaines à adénovirus
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Adénovirus humains
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Microscopie électronique à transmission
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Grippe humaine
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Sous-type H1N1 du virus de la grippe A
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Méthodes
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2013
Type:
Article