Visual detection of HIV-1 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 126-128, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-318085
ABSTRACT
<p><b>OBJECTIVE</b>A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of HIV-1.</p><p><b>METHODS</b>RT-LAMP primers were designed according to conservative sequences of HIV-1 gag gene, and their sensitivity and specificity were evaluated by the established RT-LAMP protocol with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The performance of RT-LAMP on clinical samples was compared with real-time reverse transcription PCR(qRT-PCR).</p><p><b>RESULTS</b>The RT-LAMP assay showed a high specificity, and its detection limit was 1000 copies RNA per tube. The sensitivity and specificity of this method using 43 clinical samples were 94.6% and 100%, respectively,in comparison with those of qRT-PCR.</p><p><b>CONCLUSION</b>RT-LAMP assay using hydroxynaphthol blue dye does not need expensive instruments, and offer an alternative for the rapid detection of HIV-1 with the potential to be applied in field diagnosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Sensibilité et spécificité
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
/
RT-PCR
/
Techniques d'amplification d'acides nucléiques
/
Transcription inverse
/
Réaction de polymérisation en chaine en temps réel
/
Méthodes
/
Naphtalènesulfonates
Type d'étude:
Etude diagnostique
/
Guide de pratique
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2013
Type:
Article
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