Pulsed-field gel electrophoresis typing on non-O157 Shiga toxin-producing Escherichia coli isolates / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 503-506, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-318366
ABSTRACT
<p><b>OBJECTIVE</b>To establish a database and to understand the molecular epidemiological features of non-O157 Shiga toxin-producing Escherichia coli (STEC) isolates from different animal reservoirs and patients.</p><p><b>METHODS</b>Pulsed-field gel electrophoresis (PFGE) was performed according to the PulseNet protocol with minor modifications. A dendrogram was constructed using the BioNumerics.</p><p><b>RESULTS</b>Under the PulseNet protocol, 62 PFGE patterns were obtained from 76 non-O157 STEC isolates and then divided into A to M groups. Isolates from different sources were widely distributed in different groups, but were predominant seen in certain groups.</p><p><b>CONCLUSION</b>The non-O157 STEC isolates in China were highly polymorphic. PulseNet protocol seemed to be suitable for the typing of Chinese non-O157 STEC isolates.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ADN bactérien
/
Chine
/
Épidémiologie
/
Électrophorèse en champ pulsé
/
Escherichia coli O157
/
Infections à Escherichia coli
/
Escherichia coli producteur de Shiga-toxine
/
Fèces
/
Génétique
/
Génotype
Limites du sujet:
Animaux
/
Humains
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Epidemiology
Année:
2013
Type:
Article
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