NS398 induced apoptosis in pancreatic carcinoma cell strain BxPC-3 through a COX-2-in dependent pathway / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 601-605, 2005.
Article
de Zh
| WPRIM
| ID: wpr-318855
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of the selective cyclooxygenase-2 (COX-2) inhibitor NS398 on the growth of human pancreatic tumor BxPC-3 cell strain and its possible mechanisms.</p><p><b>METHODS</b>The effect of NS398 on cell growth was assessed by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl thiazolyl blue (MTT) assay. Apoptosis was determined by fluorescence-activated cell scanning (FACS) analysis and assessment of the floating cell/attached cell ratio. Caspase-3 activation was evaluated by Active Caspase-3 Apoptosis Kit with flow cytometry. Reverse transcriptase-polymerase chain reaction analysis (RT-PCR) and Western blot were used to demonstrate expression levels of COX-1, COX-2 mRNA, and protein, as well as Caspase-3 protein in pancreatic tumor BxPC-3 cell strain.</p><p><b>RESULTS</b>Selective COX-2 inhibitor NS398 significantly decreased cell viability and induced apoptosis in pancreatic tumor BxPC-3 cell strain. The protein expression of Caspase-3 was induced by high-concentration NS398. Caspase-3 activity was strongly activated by NS398.</p><p><b>CONCLUSIONS</b>Selective COX-2 inhibitor NS398 has antiproliferative and proapoptotic potential in pancreatic tumor BxPC-3 cells. Such effect is independent of COX-2, but correlates with Caspase-3 activation.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Tumeurs du pancréas
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Anatomopathologie
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Pharmacologie
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Sulfonamides
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Cellules cancéreuses en culture
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Inhibiteurs des cyclooxygénases
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Apoptose
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Cyclooxygenase 1
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Cyclooxygenase 2
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Caspase-3
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2005
Type:
Article