Effect of ginkgolide B on L-type calcium current and cytosolic Ca2+i in guinea pig ischemic ventricular myocytes / 生理学报
Acta Physiologica Sinica
;
(6): 24-28, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-318948
ABSTRACT
With whole-cell variant patch-clamp and laser scanning confocal microscope technique, we examined the effect of ginkgolide B (GB) from ginkgo leaves on L-type calcium current and cytosolic [Ca(2+)](i) in guinea pig ischemic ventricular myocytes. The results showed that under normal conditions, at a test voltage of 0 mV, GB had no significant effect on I(Ca,L); and during ischemia, the peak Ca(2+) current reduced by 37.71%, and the I-V curve of I(Ca,L) was shifted upward. 1 micromol/L GB reversed the change induced by ischemia, a result being significantly different from those of the ishemia group (P<0.05).Under control condition, 0.1,1,10 micromol/L GB decreased intracellular calcium concentration by 10.58%, 17.27% and 16.35% (n=12, 12, 10, P<0.01-0.001), respectively. With perfusion of ischemic solution for 12 min, intracellular calcium concentration increased by 20.15%. After a 12 min-perfusion of ischemic solution containing 1 micromol/L nifedipine or 5 mmol/L NiCl2, intracellular calcium concentration increased by 18.18% (P>0.05 vs ischemia) and 11% (P<0.05 vs ischemia), respectively. After 12 min of perfusion with ischemic solution containing 1 micromol/L GB, intracellular calcium concentration increased by 9.6% (P<0.05 vs ischemia). It is shown that GB could reverse the decrease of I(Ca,L) and partially inhibit calcium overload during ischemia.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Extraits de plantes
/
Hypoxie cellulaire
/
Calcium
/
Ischémie myocardique
/
Techniques de patch-clamp
/
Canaux calciques de type L
/
Biologie cellulaire
/
Cytosol
/
Myocytes cardiaques
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Physiologica Sinica
Année:
2003
Type:
Article
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