Experimental study of periosteal osteoblasts adhesion to artificial bone scaffolds based on rapid prototype / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 248-251, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-319006
ABSTRACT
<p><b>OBJECTIVE</b>To study the biocompatibility of bone engineering scaffolds designed and fabricated by CAD and Rapid Prototyping techniques.</p><p><b>METHODS</b>Infant rat calvarias osteoblasts were isolated and expanded in vitro and the cells (2nd passage) were seeded onto scaffolds with porosity 80%, 90%, 95% at a density of 2.06 x 10(9)/L. Cell adhesion number and morphology were measured with SEM after 4 days, 10 days co-culture.</p><p><b>RESULTS</b>(1) The osteoblasts' adhesion amounts increased with culture time in three porosity group (P < 0.05), but the increase were different among three groups, 80% group was 0.35 x 10(5), 90% group was 2.84 x 10(5); (2) Through SEM observations, it showed that osteoblasts adhered to all scaffolds well.</p><p><b>CONCLUSION</b>The scaffolds designed and fabricated by CAD and rapid prototyping own a good cellular biocompatibility. The results suggest the feasibility of using such scaffold fabricating method for bone tissue engineering research and clinical therapy.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Adhérence cellulaire
/
Cellules cultivées
/
Rat Sprague-Dawley
/
Substituts osseux
/
Biologie cellulaire
/
Ingénierie tissulaire
/
Méthodes
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2004
Type:
Article
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