Effects of mitochondrial L-arginine/nitric oxide system on mitochondrial Ca2+ transport in rat myocardium / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 51-54, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-319382
ABSTRACT
<p><b>AIM AND METHODS</b>To observe the effect of myocardial mitochondrial L-arginine (L-Arg)/nitric oxide (NO) system on mitochondrial Ca2+ transport by using purified rat mitochondria and incubation of them in vitro.</p><p><b>RESULTS</b>Compared with control group, incubation of mitochondria with L-Arg (10(-4) mol/L, NO substrate) or sodium nitroprusside (5 x 10(-7) mol/L, the donor of exogenous NO, SNP) increased significantly mitochondrial NO2- (66% and 89%, P < 0.01), respectively, and decreased the Ca2+ content (40% and 54%, P < 0.01). After L-Arg or SNP treatment, mitochondrial Ca2+ uptake were decreased by 67% and 85%, respectively (P < 0.01), vs control. The rate of mitochondrial Ca2+ release decreased by 11% and 8%, respectively (P < 0.01). When L-NAME (NO synthase inhibitor) was incubated with mitochondria and the L-Arg together, it inhibited the effects of L-Arg, NO2 on the mitochondrial NO2 formation, Ca2+ content descending, and decrease of Ca2+ uptake and release.</p><p><b>CONCLUSION</b>The data suggest that myocardial mitochondrial L-Arg /NO systems take part in the regulation of cardiomyocytes Ca2+ transportation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Arginine
/
Transport biologique
/
Calcium
/
Rat Wistar
/
Nitric oxide synthase
/
Myocytes cardiaques
/
Métabolisme
/
Mitochondries du myocarde
/
Monoxyde d'azote
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2002
Type:
Article
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