Effect of blood cooling and detoxification formula and its dismantled formulae on keratinocyte proliferation induced by activated T lymphocytes / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of the blood cooling and detoxification formula and its dismantled formulae on activated T lymphocytes-induced keratinocyte proliferation and cytokine secretion.</p><p><b>METHOD</b>Rat drug-containing serum was prepared. PDB-stimulated T lymphocytes and Colo-16 cells were co-cultured, then added with the drug-containing serum, and laid aside for 24 h. The cell proliferation was detected by MTT assay. Cytokines TNF-alpha, sICAM-1 and IFN-gamma were detected by ELISA.</p><p><b>RESULT</b>The blood cooling and detoxification formula has a notable inhibitory effect on activated T lymphocyte-induced Colo-16 keratinocyte proliferation, with a significant difference between the whole formula group and the blood cooling group (P < 0.01). After activated T lymphocytes had added into Colo-16 cells for 24 hour, sICAM-1, TNF-alpha and IFN-gamma significantly increased. Though the whole blood cooling and detoxification formula, the blood cooling formula and the detoxification formula could notably reduce sICAM-1, TNF-alpha, IFN-gamma, the whole formula group showed the most significant effect.</p><p><b>CONCLUSION</b>The blood cooling and detoxification formula, as an effective traditional Chinese medicine compound for clinical treatment of psoriasis, can significantly inhibit activated T lymphocyte-induced keratinocyte proliferation and cytokine secretion.</p>