Study of pharmacokinetics of digoxin in ovariectomized rats model / 药学学报
Acta Pharmaceutica Sinica
; (12): 1603-1606, 2015.
Article
de Zh
| WPRIM
| ID: wpr-320036
Bibliothèque responsable:
WPRO
ABSTRACT
This study aims to investigate the change of plasma concentration of digoxin (DIG) in rats with ovariectomy. Twelve female SD rats were randomly assigned into ovariectomized group and sham group (n = 6). All rats plasma was collected after a single dose of 2 mg x kg(-1) DIG administrated orally, serum DIG concentration was determined by LC-MS/MS. The level of P-gp in the intestinal was analyzed by Western blotting. Pharmacokinetic calculations were performed on each individual using DAS 2.0 practical pharmacokinetic software. Compared with the sham group, C(max) of ovariectomized group decreased significantly (P < 0.01). There was no significant difference of AUC(0-t), and the level of P-gp was elevated in ovariectomized group. It was found that C(max) of DIG was significantly reduced after ovariectomy, and the change was associated with the decreased level of estrogen, which contributes to the increased level of P-gp.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sang
/
Pharmacocinétique
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Ovariectomie
/
Technique de Western
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Chromatographie en phase liquide
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Rat Sprague-Dawley
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Glycoprotéine P
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Digoxine
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Modèles animaux de maladie humaine
/
Oestrogènes
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2015
Type:
Article