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Role of connective tissue growth factor (CTGF) in proliferation and migration of pancreatic cancer cells / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 732-736, 2011.
Article Dans Chinois | WPRIM | ID: wpr-320149
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of connective tissue growth factor (CTGF) in pancreatic cancer and its influence on the proliferation and migration of cancer cells.</p><p><b>METHODS</b>The expression of CTGF in pancreatic cell line PANC-1 cells was analyzed by real-time PCR and in pancreatic carcinoma (50 cases) tissues by immunohistochemistry. The ability of proliferation and migration in vitro of PANC-1 cells was tested by MTT assay, scratch test and Boyden chamber test after the CTGF gene was overexpressed by Ad5-CTGF or silenced with Ad5-siCTGF transfection.</p><p><b>RESULTS</b>CTGF was overexpressed in both pancreatic cancer cells and tissues. Overxpression of CTGF leads to increased proliferation and migration of PANC-1 cells. The CTGF-transfected PANC-1 cells showed apparent stronger proliferation ability and scratch-repair ability than that of empty vector controls. The results of Boyden chamber test showed that there were 34 cells/field (200× magnificantion) of the CTGF-transfected overexpressing cells, much more than the 11 cells/field of the empty vector control cells; and 6 cells/microscopic field of the Ad5-siCTGF-transfected silenced cells, much less than the 15 cells/field of the control cells.</p><p><b>CONCLUSIONS</b>CTGF is overexpressed in both pancreatic cancer cells in vitro and in vivo, indicating that it may play an important role in the cell proliferation and migration in pancreatic cancer.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs du pancréas / Anatomopathologie / Protéines recombinantes / Transfection / Mouvement cellulaire / Adenoviridae / Lignée cellulaire tumorale / Prolifération cellulaire / Facteur de croissance du tissu conjonctif / Génétique Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Oncology Année: 2011 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs du pancréas / Anatomopathologie / Protéines recombinantes / Transfection / Mouvement cellulaire / Adenoviridae / Lignée cellulaire tumorale / Prolifération cellulaire / Facteur de croissance du tissu conjonctif / Génétique Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Oncology Année: 2011 Type: Article