Comparison of human papillomavirus detection and genotyping with four different prime sets by PCR-sequencing / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 40-47, 2013.
Article
de En
| WPRIM
| ID: wpr-320371
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To assess and compare the Human Papillomavirus (HPV) detection efficiency and the potential clinical utility of PCR sequencing-based technology.</p><p><b>METHODS</b>Four HPV consensus primer sets (GP5+/6+, MGP, MY09/11, and PGMY09/11) were used in order to amplify a broad spectrum of HPV types for HPV infection in 325 cervical samples and the PCR products were sequenced afterwards for the HPV genotyping.</p><p><b>RESULTS</b>The HPV-positive rate was 75.4%, of which 35.5% harbored more than one HPV genotype. A total of 36 different genotypes was found, with HPV 16 (24.1%) being the most prevalent, followed by HPV 58 (13.3%) and HPV 52 (9.6%). There were substantial to almost perfect agreements between different primer sets regarding HPV detection efficiency, with the kappa value varying from 0.751 to 0.925, MGP, and PGMY09/11 were the most effective in detecting multiple infections (P < 0.001). With each of the primer sets, a board range of HPV types could be identified, though there were several differences for a few genotypes.</p><p><b>CONCLUSION</b>The substantial agreement between PCR-sequencing and HC2 for the detection of high-risk HPV (kappa=0.761) indicated that PCR-sequencing is also suitable for routine HPV screening.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Virologie
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Réaction de polymérisation en chaîne
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Infections à papillomavirus
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Alphapapillomavirus
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Génétique
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Génotype
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Méthodes
Type d'étude:
Diagnostic_studies
Limites du sujet:
Adult
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Aged
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Female
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Humans
langue:
En
Texte intégral:
Biomedical and Environmental Sciences
Année:
2013
Type:
Article